FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128802955
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA188100.043.73555415
GCATACG205650.039.9665614
ATACGAG195050.038.6289616
CGTATCA616300.036.63017749
GCCGTAT647600.036.388847
CGGCATA214800.034.7245612
GATCGGA873400.034.1788141
CGCCGTA687900.034.1094646
GCGTCGT704650.034.0639511
AGCGTCG781500.033.51348510
ATCGGAA911550.032.7134932
CGTCGTG748850.032.15492612
CCGTATC727750.031.92652948
GTAGATC835850.031.90457331
ATCTCGG995950.031.22228435
AGAGCGT842100.031.1215238
TCGCCGT651600.030.95154245
GATCTCG987850.030.71973834
TGGTCGC669950.030.52155742
TCGGTGG898200.030.3851438
Produced by FastQC (version 0.11.5)