FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55968510
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA7972121.4243938243129932TruSeq Adapter, Index 10 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGTGGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3676700.6569229732933751TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1336750.046.5220247
TCGTATG1346300.046.21974644
CGTATGC1359800.045.73550845
CTCGTAT1360300.045.6096843
GTATGCC1393300.044.65319446
AATCTCG1337900.044.0523340
TCTCGTA1371650.043.2577842
ACGTCTG1445550.043.20424314
GCCGTCT1435700.043.1690950
ACACGTC1456300.043.10800612
ATGCCGT1450600.042.78279548
CGTCTGA1460050.042.7728215
GATCGGA638200.042.445231
ATCTCGT1399450.042.43047341
CACGTCT1489150.042.1176813
CACACGT1500100.041.9022311
CCGTCTT1493600.041.61111551
TGCCGTC1494650.041.57726749
GCACACG1566200.040.19098710
ATCGGAA1569400.039.899661
Produced by FastQC (version 0.11.5)