FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27676390
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTAC52300.020.52688635
TTACCTA56400.019.19032334
CCTTACC58350.018.73754132
CTTACCT62250.017.56362333
ACCTACA67100.016.26224736
TGCCGAC72300.014.96966622
CCTACAT72350.014.96061837
AGTTACG76700.014.3115216
GTTACGG77600.014.11729057
TACATTG79750.013.71032939
GCCGACT79700.013.66263823
TTACGGA81050.013.6249358
CGAAACG8500.013.4581033
TTGCCGA81200.013.35594521
TACGGAT83050.013.190879
GTCCGAT24950.013.1698141
CCGGTAT39450.013.1366541
TACGGTA50750.013.0908344
CGCTATG19550.013.05352929
ACGTTCG16400.012.8768016
Produced by FastQC (version 0.11.5)