FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27676390
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAT20700.09.56450912
CGTAGGG17500.09.17719537
TAGCATA38300.08.9029139
GTAGCAT41200.08.7569518
TAACACG16850.08.7500854
GTCTAAC22450.08.7245531
TCGCGAA10100.08.276933
GCGTCGA26150.08.24414711
GCGTAGG20000.08.13958936
GGTAAGC46550.07.94005166
TAGGGGA51100.07.749722539
AATCTCG51450.07.48444185
GCATAGG29400.07.48261636
GGCGTAG22550.07.41434135
GTCTAAG46300.07.31781344
TCTCGCG49350.07.26641277
CATAGGG30500.07.213155337
TACGCGT5901.9545587E-77.0846967
CGTGCGT31700.07.0091998
ATCTCGC58550.06.98907666
Produced by FastQC (version 0.11.5)