FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198922126
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG346300.026.24592843
TATGCCG343950.026.12757346
CGTATGC358900.025.2952144
CTCGTAT377000.023.89466342
GATCGGA419050.020.0022261
ACACGTC481850.019.59090813
TCTCGTA480100.019.01922641
ATCTCGT513650.017.86574640
ACGTCTG544400.017.52631815
ATGCCGT567350.016.35619447
ATCGGAA517700.016.3233092
CACGTCT593250.016.05946714
CACACGT649300.014.83407112
ACTTAGG651850.014.682487532
CACTTAG673650.014.09221831
CGTCTGA687950.013.94021316
CTTAGGC692100.013.731935533
GTATGCC674850.013.62941645
CCGTCTT676600.013.48836750
GCACACG740700.013.39464511
Produced by FastQC (version 0.11.5)