FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198922126
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA239300.032.2541615
GCATACG262000.031.07469414
CGGCATA246650.029.4201812
ATACGAG248600.028.5012716
TACGAGA352000.017.77998517
ACGAGAT370950.016.6519718
ACGGCAT474300.015.80039711
AAGACGG536500.014.13351258
GCCTAAG539150.013.92018625
AGACGGC583800.013.7249579
GGCATAC560700.013.67831313
TGCCTAA576450.012.66043524
CGTATCA200350.012.32976150
GACGGCA646950.012.10345110
CCTAAGT494850.012.09519526
ATACGCG40050.011.87578416
GCCGTAT216950.011.56569648
CGCCGTA236250.010.69796747
GAAGACG767700.010.1129867
CTAAGTG523450.09.908927
Produced by FastQC (version 0.11.5)