FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50012072
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15822803.1637961330616338TruSeq Adapter, Index 18 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCCACTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA3780030.755823513970787TruSeq Adapter, Index 18 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2118450.054.61715347
TCGTATG2124250.054.50201844
CTCGTAT2138050.053.9681743
CGTATGC2145600.053.96177745
GTATGCC2175400.053.21375346
TCTCGTA2136900.052.7621542
ATGCCGT2198000.052.58399648
GCCGTCT2203200.052.39568350
CGTCTGA2214900.052.27470815
ATCTCGT2166750.052.02507441
ACGTCTG2233900.051.88471614
ACACGTC2243300.051.69745312
TGCCGTC2239250.051.60608349
CCGTCTT2252000.051.25206451
GATCTCG2201150.051.2072140
CACGTCT2269350.051.14870513
CACACGT2289000.050.7661911
ACTGATC2252400.050.10717437
CGTCTTC2306250.050.0578852
CTTGAAA2324100.049.77535261
Produced by FastQC (version 0.11.5)