FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92449112
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT206450.018.73770942-43
CGTATCG117550.018.5567133
TATGCCG232350.016.9320546-47
CGTATGC235600.016.80721744-45
CCCGTAT157500.016.1405091
GTATCGA139400.015.9845844
ATCTCGT289500.013.64477840-41
CCGTATC173050.013.608882
CGACCGT64250.013.3106361
TCGTATG217900.012.709969542-43
TATCTCG240950.011.51963538-39
ACTAGCT355350.011.44269132-33
TAGCTTA368200.011.14499334-35
TCTCGTA252100.011.00363940-41
GCTTATC383950.010.57793136-37
TATCGAA231600.010.5731665
CTCGGGT255300.010.3623471
CTCGGAT246750.010.340541
ATCGAAG284200.010.2850586
CGCGTAT18650.010.0781711
Produced by FastQC (version 0.11.5)