FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103800400
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG134600.022.2919744
TATGCCG143150.020.86069547
CGTATGC151650.019.78585845
CTCGTAT151250.019.69879743
TATCTCG166750.017.57312840
GATCGGA183800.015.6059942
TCTCGTA193250.015.4720942
ACCGATG222100.014.30562233
ACACGTC218250.014.18314114
CGATGTA218000.014.05009135
CACCGAT237750.012.99350732
ATCGGAA224500.012.7411113
CCGATGT242200.012.70382534
ATCTCGT243200.012.56853141
ATGCCGT242200.012.55999148
AGATCGG232650.012.377291
ACGTCTG260300.012.14226616
TCACCGA284400.011.24607231
CACGTCT286300.010.97865615
CACACGT294600.010.86253713
Produced by FastQC (version 0.11.5)