FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43241009
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG105700.022.6147446
CGTATGC105500.022.0933847
TATGCCG106650.021.66161349
CTCGTAT114400.021.41494445
AGATCGG125550.020.3945621
AATCTCG143750.019.893342
ACACGTC157350.019.54774914
TCTCGTA129650.019.03088644
GATCGGA139300.018.5712322
ACGTCTG167950.018.33471916
ATCTCGT145650.017.59110843
ATCGGAA154150.016.693023
CACGTCT186400.016.68888915
CACACGT186750.016.63916213
GCACACG210400.015.21799712
CGTCTGA207500.015.177649517
TCGGAAG198000.014.4636934
ATGCCGT160100.014.05830150
GTATGCC173650.013.92850248
TATATCG37800.013.4255235
Produced by FastQC (version 0.11.5)