FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145465700
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA258100.031.1122950
GCCGTAT264400.030.29495648
CGCCGTA286200.028.0119247
GCGTCGT328000.024.69486212
TCGCCGT335600.023.8064146
TGGTCGC334200.023.70091643
CCGTATC339500.023.61277849
GATCGGA381800.022.063582
CGTCGTG370600.021.85620513
AGCGTCG386900.021.06025111
AGATCGG413400.019.9096181
GTCGCCG416200.019.61062645
AGAGCGT425700.019.4656839
GTAGATC442000.019.0146432
ATCGGAA441300.018.8396283
TCGTGTA436600.018.74121915
CGTGTAG450900.018.23829316
GTCGTGT460200.017.84073414
GGTCGCC476200.017.21262744
GTATCAT485400.016.9411651
Produced by FastQC (version 0.11.5)