FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169880094
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2449580.14419464590124373TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG607250.035.31685346
TATGCCG627600.034.23880849
CTCGTAT631650.033.73088545
CGTATGC638200.033.6947
TATCTCG650100.031.87774842
AGATCGG685800.031.5560231
TCGAACG305300.030.76597640
AGTCACG754550.028.69643629
GATCGGA760350.028.46532
ATCTCGT743550.028.0713543
ACACGTC775650.028.00187314
GTCACGT775750.027.97054530
ACGGTAA342800.027.47046544
TCTCGTA782350.026.73143844
CGAACGG354400.026.59118541
AACGGTA355750.026.52946743
ACGTCTG824900.026.41001316
CGGTAAC364950.025.97231345
TCACGTG846250.025.77553431
ATGCCGT858700.025.03628250
Produced by FastQC (version 0.11.5)