FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168879955
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8257150.488936061121049TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5358340.3172869154305495TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG1139300.041.786171
TCGTATG1903650.036.5274245
CTCGTAT1906300.036.2410644
TATGCCG1920950.036.0620648
CGTATGC1932700.035.96100646
AGTCACG2007100.034.82815628
ACACGTC2024550.034.7940613
GTCACGT2008250.034.7798329
ACGTCCG2026350.034.50484532
GATCGGA2071950.034.2151831
ACGTCTG2070000.033.98084615
CGCACAT1965850.033.8636737
TCACGTC2078150.033.6122130
CACGTCT2113350.033.30759414
ATCTCGT2006500.033.1197942
GTCCGCA2136800.032.6382834
ATGCCGT2110500.032.5959749
TTGAACG509750.032.541357
GCCGTCT2125500.032.2164651
TCTCGTA2064900.032.17510643
Produced by FastQC (version 0.11.5)