FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168879955
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA2182250.12921900648303702Illumina Single End PCR Primer 1 (100% over 57bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAA1887330.1117557142882943Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG1047550.044.523831
CGTATCA1809250.037.5074649
GCCGTAT1835950.037.05282247
GATCGGA1939550.035.43531
CCGTATC1917000.035.3610248
TCGCCGT1930400.035.1892945
GCGTCGT1959150.034.71142611
TGGTCGC1981600.034.35423742
CGCCGTA1984350.034.27741246
GTCGCCG2010950.034.03081544
GTATCAT2008600.033.952450
AGAGCGT2015950.033.8470048
GTAGATC2079300.033.21236431
CGTCGTG2067800.032.9135512
GGTCGCC2088500.032.7873343
TCGTGTA2051700.032.71668214
GTCGTGT2087150.032.38089413
TCTCGGT2135800.032.21930336
GAGTGTA2126800.031.87308927
TAGATCT2171700.031.7912432
Produced by FastQC (version 0.11.5)