FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116946962
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA3003220.25680188254911657TruSeq Adapter, Index 7 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2308270.19737750861796652TruSeq Adapter, Index 7 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG668500.039.01891
TCGTATG1138700.037.63590643
CTCGTAT1148350.037.0911742
TATGCCG1148700.037.01844446
CGTATGC1163250.036.7368644
ACACGTC1231300.035.70577613
TCTCGTA1188800.035.68348341
GATCGGA1249150.035.4625631
ATCTCGT1231500.034.50761840
ACGTCTG1274050.034.47235515
ATCGGAA1287600.034.357482
CACACGT1306700.033.8714512
ATGCCGT1270900.033.45529647
CACGTCT1324550.033.22329314
GCACACG1380100.032.17673511
CATCTCG1354950.031.60863939
GCCGTCT1348450.031.57989949
GTATGCC1359150.031.48500345
CCGTCTT1353050.031.47727250
CGTCTGA1403750.031.32657416
Produced by FastQC (version 0.11.5)