FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162443654
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2810460.1730113753781973TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG701850.040.0545546
CGTATGC736750.038.28454247
TATGCCG740400.038.07782749
CTCGTAT722050.037.45830545
AGATCGG788900.036.691511
GATCGGA817250.035.5032462
ACCCGTC822850.034.87647633
ACACGTC847700.034.1039914
CGTCCCG851900.033.6228736
TCTCGTA796650.033.4908144
TCCCGAT837550.033.2091238
ACGTCTG882700.032.85380616
ATCGGAA908800.031.8770643
CCCGATC850350.031.70101239
CACCCGT919000.031.23782332
GTCCCGA918550.031.04373637
CCGATCT868500.030.87036540
CACGTCT945600.030.62822215
ATGCCGT922100.030.59705450
ATCTCGT870250.030.54062543
Produced by FastQC (version 0.11.5)