FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162443654
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA547850.050.8472450
GCCGTAT591700.047.28508448
CGCCGTA606950.046.2681247
GCGTCGT667250.042.85250512
CCGTATC656400.042.64490549
GATCGGA691250.041.943682
TCGCCGT671200.041.2329446
TGGTCGC681100.040.78521343
TCGTGTA711700.039.88963315
CGTCGTG729750.039.3147613
AGCGTCG740750.038.7356111
GTCGTGT737500.038.4853614
GTAGATC752600.038.0004932
CGTGTAG753550.037.7745216
ATCGGAA784850.036.8668673
AGAGCGT787600.036.482559
GGTCGCC777800.036.25644344
GTCGCCG779300.036.25350645
AGATCGG798150.035.9120331
GTATCAT789350.035.69135351
Produced by FastQC (version 0.11.5)