FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269822812
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA4076960.1510976766486297TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG917300.039.53110533
TATGCCG930800.038.6938949
CTCGTAT934700.038.06659345
TCTCGTA981400.036.0850344
CGTCCGC1020300.035.51730334
TCGTATG1036300.034.89692346
AGATCGG1061200.034.5304071
ACACGTC1087100.033.6476814
GATCGGA1102650.033.2667432
CGCACAT1084450.033.071738
TCACGTC1104600.032.96525231
ATCTCGT1089700.032.66090443
CGTATGC1126550.032.15373247
GTCCGCA1143900.031.71569435
AGTCACG1165650.031.19944229
ATGCCGT1188450.030.45911850
GTCACGT1224450.029.80599830
ACGTCTG1231450.029.7821716
TCCGCAC1233900.029.44682136
CACACGT1282200.028.70544813
Produced by FastQC (version 0.11.5)