FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269822812
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA703500.050.66967850
CGCCGTA723600.049.22290447
CCGTATC804950.044.46834649
GCCGTAT808400.044.1886548
TCGCCGT807700.043.71801846
TCGTGTA918500.039.44441615
TGGTCGC934100.038.0269843
GTCGTGT954700.038.02198814
GTCGCCG983750.036.43948445
GATCGGA1007050.036.2480552
GTGGTCG998550.035.7193442
GGTCGCC1007950.035.64544744
CGTGTAG1055850.034.66693516
AGATCGG1056650.034.360971
AGAGCGT1079100.033.7273949
GATCTCG1089150.033.63902735
GCGTCGT1091200.033.2603712
GAGCGTC1119200.032.58255410
CGTCGTG1140850.031.8884413
GTAGATC1158700.031.49922232
Produced by FastQC (version 0.11.5)