FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125709094
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT273450.015.45774242-43
CGTATGC309950.013.88690744-45
TATGCCG308750.013.84284946-47
CGTATCG99800.012.7850023
TCGTATG274750.011.043838544-45
ATCTCGT398200.010.94390440-41
CGATGTA396200.010.8360334-35
CCCGTAT179100.010.808521
ACCGATG409300.010.77552332-33
TATCTCG290650.010.61636940-41
CGCGAGT120900.010.1821531
CCGGGAT356350.09.6120361
CGCGTAT34550.09.2475241
GTATCGA147650.09.1182294
TCTCGTA347800.09.027442-43
CTCGGAT367000.08.872211
CCGGAAT280150.08.8721671
CGACGAT139450.08.7266161
CGCGGAT149400.08.7115171
AGATCGG1016450.08.64249694
Produced by FastQC (version 0.11.5)