FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71904391
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1694340.23563790422757355TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA523600.038.7704351
TCGTATG544950.037.3733445
CGTATGC554400.036.7307446
TATGCCG553350.036.71315848
CTCGTAT522700.036.6477644
ATCGGAA561850.036.080662
ACACGTC610050.034.37906613
TCTCGTA552800.034.3028343
TCGGAAG596400.034.0309753
AATCTCG562350.034.01975641
ATCTCGT572400.033.1878842
ACGTCTG633950.033.02343815
CACACGT655750.032.07257512
CACGTCT657000.031.9484614
CGGAAGA665050.031.8142624
GCACACG666650.031.67753411
ATGCCGT642150.031.378449
GCCGTCT650800.031.03007751
CCGTCTT663950.030.45707152
GTATGCC671850.030.43285647
Produced by FastQC (version 0.11.5)