FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71904391
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT316500.032.51639647
CGTATCA317800.032.21053749
AGCGTCG382600.030.17673510
GCGTCGT357850.030.00200311
AGATCGG254550.029.7822861
CGCCGTA349050.029.45620746
TCGCCGT338400.028.588845
GATCGGA437600.028.2484191
GTCGCCG362900.028.21783444
CGTCGTG380800.028.1483312
AGAGCGT411200.028.1362958
TGGTCGC330350.027.79180342
CCGTATC371550.027.79126748
GTAGATC426200.026.29342331
GAGCGTC439950.026.2042949
ATCTCGG469000.025.98806835
ATCGGAA482500.025.6998042
GATCTCG469150.025.626934
AAGAGCG478650.025.1082847
GTATCAT418600.024.76735350
Produced by FastQC (version 0.11.5)