FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80270457
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG183800.027.4960846
CTCGTAT183650.026.3559945
TATGCCG201650.024.99660749
CGTATGC202950.024.91867647
AGATCGG205800.021.9048841
TCTCGTA219850.021.84109344
GAAACGA248500.021.0305337
GTCACGT246050.020.98154830
TGAAACG248900.020.89560536
AGTCACG252300.020.67000829
GATCGGA225100.020.1236482
AAACGAT252700.020.0974738
ACACGTC261850.020.0582914
ATCTCGT249200.019.43930643
ACGATCT251400.019.36629140
CGTGAAA274650.019.26870334
ACGTCTG274050.019.12695916
TCACGTG284650.018.39549631
CACGTGA287850.018.23941632
AACGATC265450.018.22253639
Produced by FastQC (version 0.11.5)