FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80270457
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT133350.034.31474348
CGTATCA148450.031.15434850
GCGTCGT166250.029.03400612
TCGCCGT160300.028.02369346
CGCCGTA171350.026.6234347
CGTCGTG190900.025.61773313
GATCGGA192150.025.331712
TGGTCGC182300.025.00973943
AGCGTCG199500.024.5119411
CCGTATC199000.023.63034249
GTCGCCG205850.023.162745
AGATCGG209800.022.8069441
AGAGCGT216200.022.5795259
GTAGATC232050.021.39560732
ATCGGAA235800.020.7010573
TCTCGGT242650.019.95938137
GATCTCG255000.019.60562735
TCGGTGG251150.019.15143639
ATCTCGG267200.018.8673536
GAGCGTC264350.018.66656910
Produced by FastQC (version 0.11.5)