FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63555560
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10600611.6679280302148232TruSeq Adapter, Index 15 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGGCATGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5460890.8592308839698682TruSeq Adapter, Index 15 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1741500.044.41396347
TCGTATG1751100.044.1990244
CTCGTAT1764250.043.73657243
CGTATGC1789300.043.2771345
GTATGCC1819400.042.5388746
TCTCGTA1782000.042.178142
GATCGGA935000.042.118351
GCCGTCT1836750.042.04171450
ATGCCGT1839350.042.01558748
ACGTCTG1851550.041.81257214
ATCTCGT1803000.041.729241
CGTCTGA1855850.041.697315
ACACGTC1861800.041.64106412
TGCCGTC1880700.041.1132249
CACGTCT1891550.041.01425613
CCGTCTT1883350.040.9904851
GATCTCG1851450.040.6185740
CACACGT1914050.040.56742511
GCATGAT1928650.039.15513236
GCACACG1985700.039.14874310
Produced by FastQC (version 0.11.5)