FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68829706
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1280120.1859836507219717TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1155130.16782434026378087TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG283350.040.263741
GATCGGA489150.030.1054211
TCGTATG477950.028.54169845
CTCGTAT470300.027.94449644
CGTATGC488150.027.85098646
TATGCCG486850.027.79284148
ATCGGAA532800.027.6636052
ACACGTC534300.027.3656613
ACGTCTG549900.026.73414415
TCTCGTA502350.026.2508543
AATCTCG520500.026.21467641
CACACGT567500.025.92085512
ATCTCGT515400.025.67288842
TCGGAAG578550.025.6012253
CACGTCT582700.025.18139614
GCACACG590000.025.0667711
CGGAAGA607400.024.4306164
GTATGCC594950.023.01570347
ATGCCGT581100.023.01434349
GCCGTCT584500.022.99338551
Produced by FastQC (version 0.11.5)