FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88661784
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT119150.030.39900648
CGTATCA118600.030.01642250
GCGTCGT150850.024.64284512
TCGCCGT152700.023.58420246
AGCGTCG165000.022.63365611
CGCCGTA160300.022.61668447
CGTCGTG167350.022.25431413
GATCGGA179150.021.1246472
AGAGCGT192850.019.5394339
TGGTCGC185850.019.52592743
CCGTATC197550.018.49236149
CGTATCG76800.018.2384533
AGATCGG203250.018.2290821
GTCGCCG202300.018.12595245
GTCGTGT228500.016.40320814
GAGCGTC231600.016.34669310
ATCGGAA231150.016.2538853
GTAGATC228300.016.15424332
GTATCGA89850.016.0507324
TCGTGTA235400.015.99209715
Produced by FastQC (version 0.11.5)