FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95381736
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1030480.10803745488549296TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT271750.023.9787942-43
TATGCCG305500.021.5530746-47
CGTATGC307950.021.38072444-45
TCGTATG288300.019.06686242-43
ATCTCGT374600.017.70691340-41
TCTCGTA336800.016.39676940-41
ACTTAGG465400.014.50005232-33
GATCGGA752300.014.31516551
ATCGGAA755350.014.2666952
TTAGGCA475700.014.15132134-35
TCGGAAG792350.013.7595923
CACGTCT505450.013.4104714-15
ATGCCGT435400.012.905498546-47
CCGTCTT524100.012.83763150-51
CACACGT537600.012.65210612-13
TCACTTA538750.012.64784530-31
CGGAAGA883850.012.6110934
ACACGTC441150.012.60964412-13
ACGTCTG459550.012.20155114-15
CGTCTGA566550.012.15439816-17
Produced by FastQC (version 0.11.5)