FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117041596
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT340500.022.98346942-43
TATGCCG354500.022.2221746-47
CGTATGC375550.021.06305944-45
ATCTCGT452200.017.59522840-41
CACGTCT591950.013.68185514-15
TCGTATG348750.013.403398542-43
CCGTCTT610500.013.028250-51
CACACGT635050.012.80496712-13
CGTCTGA662000.012.37208416-17
CGTATCG128250.012.1078113
TGCCGTC685750.011.88562448-49
GTATCGA140650.011.6740624
TCTCGTA408000.011.44471340-41
CCCGTAT168600.011.3710781
TAATCTC713250.011.29364738-39
AATCTCG427000.011.22332638-39
CGACCGT86900.010.8686441
GATCGGA846500.010.5413691
ATCGGAA854600.010.4909792
CTTGTAA795350.010.42778934-35
Produced by FastQC (version 0.11.5)