FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181266290
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG362200.018.81349843
CTCGTAT369950.017.92743742
TATGCCG377900.017.3462746
CGTATGC414300.016.27866644
GATCGGA437000.016.2344381
AATCTCG449000.015.48827739
TCTCGTA440400.015.44902341
ACACGTC491650.015.05632913
ATCTCGT506750.013.77847740
ATCGGAA543050.012.97752
ACGTCTG578600.012.85457915
ATGCCGT552400.012.13273347
CACGTCT627700.012.1111414
CGTCTGA624300.011.99769416
CACACGT630350.011.976130512
CTCGAAT343100.011.3287371
GCACACG711150.010.84662511
CCGTCTT635000.010.59862450
GCCGTCT635150.010.57959149
GACCAAT711050.010.48882535
Produced by FastQC (version 0.11.5)