FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181266290
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATA257650.030.49868612
ATACGAG234150.030.20851916
CATACGA271500.027.03791415
GCATACG306900.026.49241414
TACGAGA309850.021.06291617
CGAGATT335500.018.74306319
ACGAGAT366250.017.718818
ACGGCAT446000.017.0704111
AGACGGC489000.016.6695589
AAGACGG494300.016.321338
GGCATAC525500.015.5185713
GACGGCA527250.014.69163210
CGTATCA196400.014.07115350
CGCCGTA222700.012.08867347
GCCGTAT230700.012.02773648
GAAGACG712500.011.4065437
CTCGAAT342850.011.130261
ATACGCG39000.010.12574316
CCGTATC274850.010.10618649
CGGAAAT318750.09.7572331
Produced by FastQC (version 0.11.5)