FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178993682
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT280000.027.10383435
TACGTAA288400.026.56841737
ACCGTAC295250.026.14172633
TCGTATG287950.025.9438646
CCGTACG299100.025.7111234
TATGCCG298000.025.26805749
CGTAATC297600.024.74444639
GTACGTA314800.024.32923736
CTCGTAT307900.023.99651545
CGTATGC314350.023.85390747
ACGTAAT322550.023.4202938
CACCGTA407150.019.3291832
GATCGGA380200.019.1680622
TCTCGTA390450.018.96791544
AATCTCG403500.018.88516642
ACACGTC443100.017.91370814
AGATCGG408700.017.7234081
ATCTCGT445050.016.94055443
ACGTCTG515250.015.61582616
ATGCCGT493900.015.25989250
Produced by FastQC (version 0.11.5)