FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178993682
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA245850.029.63113250
GCCGTAT257250.028.55405848
CGCCGTA288700.025.38987447
GCGTCGT328650.022.87824212
CCGTATC330750.022.46024349
GATCGGA358750.021.2648072
TCGCCGT347000.020.75454946
CGTCGTG387750.020.1405313
AGCGTCG386700.019.56103911
TCGTGTA400950.019.10246815
CGTGTAG431550.018.15316416
GTCGTGT425900.018.05726214
TGGTCGC405000.017.8743
AGATCGG424500.017.7252391
AGAGCGT456050.016.872689
ATCGGAA472200.016.17753
GTCGCCG465950.016.05789645
GAGCGTC491550.015.58871610
GTAGATC521500.014.81964832
GGTCGCC528900.014.15380644
Produced by FastQC (version 0.11.5)