FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58620477
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA17274292.9468013370140267TruSeq Adapter, Index 11 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6192031.0562913024402718TruSeq Adapter, Index 11 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2135000.059.2645231
TCGTATG2630450.048.89813645
TATGCCG2633300.048.82433348
CGCTATG2638800.048.7340934
CGTATGC2647800.048.5607546
CTCGTAT2648700.048.47130244
CCGCTAT2654750.048.42959233
ACCGCTA2656900.048.40594532
TATCTCG2632150.048.26745641
GTATGCC2681350.047.96612547
TCTCGTA2649700.047.95956443
TCACCGC2690700.047.78497730
ATCTCGT2674350.047.5342442
GCCGTCT2720700.047.24571251
ATGTATC2693750.047.17384338
CACCGCT2732450.047.1129331
ACGTCTG2750500.047.04575315
GTATCTC2702650.047.03637740
ACACGTC2760400.046.9882613
CTATGTA2749150.046.83183736
Produced by FastQC (version 0.11.5)