FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163345635
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA3637130.22266465828731816TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2651140.16230246985173494TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG795500.038.333081
TCGTATG965850.031.25760744
CGTATGC984300.030.69622845
TATGCCG985650.030.60255847
CTCGTAT982000.030.5222543
TATCTCG1005100.028.95084840
GATCGGA1089500.028.058322
ACACGTC1113300.027.44307314
TCGAACG339900.027.20001240
ATCTCGT1083550.026.95541
TAGCTTA1121450.026.89704935
ATGCCGT1142750.026.28994248
ACGTCTG1165350.026.19897816
TCTCGTA1123400.025.9284442
TTGAACG381650.025.6128867
CACTAGC1201550.025.27637332
GCTTATC1160350.025.1655237
CACGTCT1216900.025.09771515
ACTAGCT1217450.024.96601533
ACGGTAA376150.024.67804344
Produced by FastQC (version 0.11.5)