FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163345635
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAA1649450.10097912931680114Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG794000.037.9647031
CGTATCA934950.031.96177550
GCCGTAT954200.031.2916848
CCGTATC1010500.029.67080749
CGCCGTA1030600.029.0359547
TCGCCGT1042350.028.7769546
GATCGGA1070850.028.2123872
GCGTCGT1086950.027.55011212
TGGTCGC1106950.027.34113543
TCGTGTA1106950.027.0768615
TCGAACG325700.027.0199840
GTCGCCG1123750.026.88124545
GTCGTGT1129700.026.56554614
GTATCAT1141650.026.39529451
CGTCGTG1138000.026.36019913
AGAGCGT1145500.026.2852979
CGTGTAG1177500.025.66215916
GGTCGCC1176950.025.62496644
AGCGTCG1209450.024.89054311
TTGAACG376950.024.8809437
Produced by FastQC (version 0.11.5)