FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37609186
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGGCGTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA6365971.692663595537537Illumina Single End PCR Primer 1 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA905000.061.6007258
GATCGGA1554550.061.405691
GCCGTAT968850.060.97943559
CGTATCA957100.060.6159261
TCTCGGT1279600.059.8040248
CCGTATC965750.059.49084560
GTAGATC1317900.059.403743
TCGCCGT975950.059.35859357
TGTTGCG1585300.059.32791530
GTCGCCG1041200.059.02464356
TAGATCT1335100.058.90466744
TTGCGGC1607300.058.41004632
GTTGCGG1613100.058.3334131
CGTGTAG1637850.058.24215
GCGTCGT1643350.058.1466311
GTATCAT1013450.058.06471362
GTAGGGA1640100.057.96096818
GGTCGCC1054800.057.9067155
GATCTCG1306450.057.8303646
ATCATTA1036200.057.7511964
Produced by FastQC (version 0.11.5)