FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71654115
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCCAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA18507152.582845381594623Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAAGTCCAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA5498770.7674046354490597Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2498200.052.16265557
GCCGTAT2502200.052.06435858
CGTATCA2509400.051.8883860
GTAAGTC2545450.051.48147630
TCTCGGT2557000.051.3169547
GTAGATC2563500.051.23027442
CCGTATC2543950.051.20536859
TAGATCT2575750.050.9574843
CGTGTAG2586400.050.82607314
GTGTAAG2570150.050.82106828
GTATCAT2567800.050.73089661
TGTAAGT2576350.050.72630729
TAAGTCC2589300.050.6482731
AGTGTAG2602650.050.4565139
GGTCGCC2598300.050.41459354
GTAGGGA2608100.050.40587217
GAGTGTA2591900.050.4039226
GCGTCGT2611600.050.38559710
TGGTCGC2599750.050.3678953
AGTGTAA2602700.050.1854927