FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36888457
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG594960.16128622566132272No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC479850.13008134224752205No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG469560.12729185175731259No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG431090.11686311520159275No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC426620.11565135402654549No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG407350.11042749768579369No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA383460.1039512170433152No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT380590.10317319588618196No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT45500.030.0606121
TATCGCG16700.029.64074522
GGTGGCG194300.028.7635731
GGCGATC51250.026.0651652
TGGCGCG221000.025.1935633
CGACGTA12050.024.43620735
GGCGCGT231550.023.7619634
GGTCGAC133850.023.3863351
GTGGCGC250650.022.325922
TAGGGGG77800.021.42571838
GCGCGTG261600.021.085885
GCGATCT64250.020.9508823
CGCGTGC263300.020.7914316
TATAGGG56600.020.5065336
AGGTCGA98650.020.415256
CTAGCCT98900.020.0155162
ATAGGGG69150.019.59312837
ATATCGC26700.018.5388921
GTCGACC168200.018.4969772
CTATTAG109300.017.95404611
Produced by FastQC (version 0.11.5)