FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005432258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56764309
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG10372321.8272608585792878No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT4638700.8171860244084008No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC3630700.63960965331226No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCC3424640.6033086741177454No Hit
CGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATA3022710.5325018578135075No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT2918210.5140924026750682No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCG2882040.5077204410257157No Hit
CGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAA2440360.4299109850874781No Hit
GTCAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCC2290930.40358634507468416No Hit
CTCGTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA2235540.39382845301613734No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA2188920.3856155458529408No Hit
CCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGAT2023080.35640000479879No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC1931830.340324762871684No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA1919310.33811915159576766No Hit
GTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCG1881560.3314688460314033No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG1868120.3291011610834547No Hit
CGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCAC1635350.28809476038896203No Hit
GGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG1564400.27559570926865334No Hit
CACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCA1547940.27269599987555565No Hit
GTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGAT1526140.2688555585165319No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC1525890.2688115167578275No Hit
CGTGAGGCCCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAA1497460.2638030879579632No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA1455190.2563565073962232No Hit
CTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGCGCCGAGA1403760.24729623679555407No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA1385480.24407590339908833No Hit
CACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCA1383710.24376408774746117No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG1367710.24094541519037957No Hit
CTCAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGG1323250.23311302882238907No Hit
CGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGG1292610.22771527087557783No Hit
CTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGG1262940.22248839495253964No Hit
CCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGAC1239850.21842069811860124No Hit
CACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCT1238130.21811769081871496No Hit
GGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGT1209110.21300532346830822No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT1186080.2089481966564589No Hit
CGCAGGGCCGGCGGACCCCGCCCCGGGCCCCTCGCGGGGACACCGGGGGGGCGCCGGGGGCCTCCCACTTATTCT1169860.2060907673517174No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGATCAATGTGTCCTGC1167700.2057102465565114No Hit
CATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCAC1167140.20561159301701357No Hit
CACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC1158430.20407717814375226No Hit
CTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCAATCGGT1102710.1942611509637156No Hit
CGAAACCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGT1079160.19011241729376113No Hit
CAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGC1072450.18893033649013502No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1041010.1833916449154697No Hit
CGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCG1039170.1830674975714053No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA1008530.17766973962459404No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG1007550.1774970959304728No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAACATGCC1002410.1765915973715103No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG982120.17301716823506122No Hit
GTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAA974030.17159197692338685No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT973080.17142461824031013No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG958710.16889309794998122No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG950390.16742738822029876No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCAT937620.16517773518567805No Hit
CAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCAG904780.15939240976226804No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTA886500.15617207636580233No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC874440.15404750192590208No Hit
CAAGTTCGACCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACT866170.1525906005479605No Hit
GGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCA866100.15257826885552328No Hit
CAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCT845810.1490038397190742No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAAC837380.14751875161556183No Hit
AGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCT836290.14732672954761064No Hit
CCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAAT820260.1445027719794845No Hit
CTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGC817140.1439531308308536No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGC815790.14371530533384982No Hit
CTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGA815770.1437117819931535No Hit
CCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCC804670.1417563279066781No Hit
CTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCA796670.14034699162813732No Hit
TGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCAC791130.13937102625524783No Hit
CGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACG784760.1382488422434597No Hit
CTCCGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGC784740.13824531890276334No Hit
CAAAAGGTCAGAAGGATCGTGAGGCCCCGCTTTCACGGTCTGTATTCGTA780490.13749660900478855No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT777930.13704562139565551No Hit
ACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCC775590.13663339053418233No Hit
ACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTC774880.1365083119394618No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATT765860.13491928528540706No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT765650.13488229020809536No Hit
CTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG759410.13378300791083356No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA754120.13285108429664844No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGT733000.1291304365213007No Hit
CTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCA730560.12870058895634578No Hit
CCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTATCGTTCCGCCTGGGCG730310.12865654719764139No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCTTGG729890.12858255704301802No Hit
CTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTCTTCCGTACGCCACA723610.12747622806436346No Hit
CTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCC718680.12660772458271272No Hit
CAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGA713850.1257568378045437No Hit
CAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACAAGCCAGTTATCCC713560.1257057493644466No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTA711630.12536574698724862No Hit
CATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACT705480.12428231972312039No Hit
CGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCCA697950.12295578195094387No Hit
GCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAA697850.12293816524746211No Hit
CTCGATCAGAAGGACTTGGGCCCCCCACGAGCGGCGCCGGGGAGCGGGTC692230.1219481065117872No Hit
CTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGC690540.12165038422294544No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC686580.12095276276506775No Hit
CGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT686050.1208593942366144No Hit
GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA682130.12016881946012943No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG679270.11966498174055108No Hit
CCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCCTT672400.11845471421135419No Hit
CTCGCGGCGCCCATCTCCGCCACTCCGGATTCGGGGATCTGAACCCGACT672400.11845471421135419No Hit
CTTGTGTCGAGGGCTGACTTTCAATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGA672170.11841419579334614No Hit
TGAAAATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGG671210.11824507543992124No Hit
CTTCTTTCCCCGCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGG669110.11787512466680428No Hit
CCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTG664610.11708237301012508No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTG658050.11592671726172162No Hit
CTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTG648260.11420204199085733No Hit
GGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGAC648140.11418090194667921No Hit
CCGTCTTCTCAGCGCTCCGCCAGGGCCGTGGGCCGACCCCGGCGGGGCCGATCCGAGGGCCTCACTAAACCATCC646640.11391665139445281No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCA640900.11290545261459979No Hit
CCGGGATCGGTCGCGTTACCGCACTGGACGCCTCGCGGCGCCCATCTCCG634470.11177269858072261No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA634070.11170223176679558No Hit
CTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAA619560.10914604809159219No Hit
GTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGAC616550.10861578531679124No Hit
CGGGATTCTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC614620.10827578293959325No Hit
CAGCGAGGGAGCTGCTCTGCTACGTACGAAACCCCGACCCAGAAGCAGGT610310.1075165030195294No Hit
CTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAA608100.1071271738725825No Hit
CCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTATTAGTGGGTGA600120.10572136093473805No Hit
CTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAA596800.10513648637914363No Hit
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC590670.10405658245571175No Hit
CACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCA587100.10342766614141292No Hit
CTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAA583480.10278994147537318No Hit
TTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGC582930.10269304960622352No Hit
CGCTCCACCAACTAAGAACGGCCATGCACCACCACCCACGGAATCGAGAA579270.1020482782587911No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC576300.10152506216538283No Hit
CGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTATATCAACCAACAC575610.10140350691135867No Hit
CCCCGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCG569440.10031655630653409No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)