FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32032324
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1959480.6117195867524317No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG918830.28684462607208894No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG766590.2393176342746783No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT637600.19904893569383228No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT575400.17963105018543143No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT549660.17159541717922186No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT529670.16535484593624866No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT514130.1605034964056932No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA495200.15459384089646447No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA460120.14364240321744998No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT456160.14240615198572543No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG437760.1366619543433689No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG436250.1361905555151103No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC432200.1349262076644829No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT390570.1219299604986513No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA378060.11802453047115781No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG353750.11043532152084876No Hit
GGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTG341720.1066797401275037No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA334850.10453503155125429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG33500.036.877742
CGTACGG33900.036.4426043
ACCGCGT56500.031.7884546
CCGCGTT57500.031.1101077
AGACCGC59600.029.9603164
CGCGTTC61300.029.2944748
GGCGTAC46850.028.445271
GTACGGA47600.026.1720964
CAATACG69850.025.77546331
TTGCACG279650.025.6362048
TACGGAG71600.025.25040234
GCTACCT287150.024.5430161
ATACGGA74150.024.47304333
CTACCTT293050.024.0895882
AATACGG75150.024.05215832
TACGGAA51800.023.9836445
TGCACGG302750.023.6570689
GAACGAT76850.023.54299245
ACGGTTA306900.023.22413812
CGGTTAG307800.023.21254513
Produced by FastQC (version 0.11.5)