FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32032324
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG554810.1732031681497727No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA541290.1689824316212586No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG486500.15187783440252417No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT452980.14141340478449205No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGT398270.1243337823381157No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG375560.11724406883496807No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG372270.11621698132174238No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT370700.11572685141421522No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC365770.11418778106764904No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA355170.11087862373020453No Hit
CCCAAACCCACTCCACCTTATTACCAGACAACCTTAACCAAACCATTTAC341640.10665476535514563No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT333130.10399807394555574No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA330030.10303030151668047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG104050.039.1621538
CGGGTAA23350.039.0193549
TATAGGG76500.035.9504236
ATAGGGG87750.034.66285737
GCGGGTA27750.032.06508648
GGGCGAT40600.030.5021081
GGCGATC44300.027.9453092
CGGTGGC181100.027.5817361
GGTGGCG254000.026.325161
ATATAGG83100.026.25555635
GGTCGAC102000.023.298581
GCGGTAC105350.023.24275470
TGGCGCG298000.022.4247263
AGAGCGG64950.021.5603248
GCGATCT60200.020.6162433
GGCGCGT329400.020.2105334
GTGGCGC335250.020.0003992
AAGGGGG324750.019.89747868
CGGGTGA67150.019.78258149
TATCGCG10400.019.28608122
Produced by FastQC (version 0.11.5)