FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14000318
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN169507412.107396417709941No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1140930.814931489413312No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG291420.2081524148237204No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA255390.18241728509309574No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA221460.15818212129181639No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG220230.15730356981891414No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG215730.15408935711317415No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT175360.12525429779523578No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC156070.11147603932996379No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT152520.10894038263988004No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC148030.1057333126290417No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG144740.10338336600640072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG101350.042.7640081
ACCGCGT20250.034.9443636
CCGCGTT21550.032.9958577
AGACCGC22050.031.465584
CGACTAT12750.031.13685817
CGCGTTC23700.030.1482058
CCGACTA13500.029.40559616
CAATACG26350.027.01773331
ATACGGA27050.026.71152733
GCGTACG8700.026.5837462
AATACGG28000.025.67497832
TACGGAG27700.025.59299334
GCGTTCT29950.024.2025979
CGTACGG9750.023.72043
TCGAAGT19800.023.69933770
CAAGTCG17200.023.49798828
AGTCGTC16650.023.45261230
GAACGAT30050.023.10383645
TATCGCG12150.023.0116739
GTCGGTT7800.022.5805231
Produced by FastQC (version 0.11.5)