FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39300591
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG566190.14406653579331669No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA465570.11846386737542955No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC462910.117787032770067No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA427360.10874136727358628No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC424720.10806962165021895No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT398550.1014106887094904No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG397120.1010468264968331No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGCG241950.027.8210491
GGGCGAT46900.025.0282061
GGCGATC48800.023.833292
TGGCGCG288300.023.1309073
GGCGCGT312250.021.2870924
CGGTGGC171800.021.2029081
GTGGCGC333050.020.2150442
GGTCGAC48900.019.6143421
GCGCGTG350500.019.0836435
CGCGTGC359150.018.4243876
GCGGTAC103250.017.84381970
TCGCACG25200.017.58458327
GCGTGCC391900.017.0012027
CGCACGA27050.016.38221228
CTAGCCT129950.016.3552282
CGTGCCT427650.015.4994628
CGATCTG74650.015.3886214
GCGATCT79300.015.1866383
GTCGACC64300.015.0195792
CGCGAGT30550.014.72968213
Produced by FastQC (version 0.11.5)