FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35505229
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG676670.19058319550621683No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC542190.1527070843565042No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG528540.14886258021318494No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC514730.14497301228503554No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG509310.14344647657391535No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA423740.11934580114945886No Hit
GAACAAGTTACCCTAGGGATAACAGCGCAATCCTATTCTAGAGTCCATAT399380.11248483990907367No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG397210.11187366232731522No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGT375510.10576188650973072No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG374880.10558444785696214No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC371250.10456206323862889No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC357060.10056546882150795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGGG51050.028.09103436
GGTGGCG193300.026.8118341
ATAGGGG64200.025.57748837
TATCGCG16850.023.82779722
GGTCGAC162450.023.655921
TAGGGGG86950.023.5495938
TGGCGCG230250.022.3302633
CTAGCCT83700.021.7050972
GCGGTAC136550.021.49604870
GGCGCGT251450.020.392684
GGGCGAT35850.020.3765131
GTGGCGC255850.020.301272
CGACGTA15000.020.0877435
AGGTCGA113900.020.019066
GGCGATC36350.019.8009852
CGGGTAA16400.019.06837849
GTCGACC204500.018.6473082
CGGTGGC153800.018.5034221
ACATAGC78300.018.04182270
ATATAGG62600.017.9809335
Produced by FastQC (version 0.11.5)