FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20333180
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG837560.4119178603641929No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT561460.2761299511438939No Hit
GCTACCTTTGCACGGTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTG435430.21414751652225572No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT335780.16513895022814926No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC334480.16449960114453321No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC313250.15405853880209588No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT308690.15181589893956576No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA307470.1512158944149415No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT302410.1487273510587129No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT292310.14376010048600366No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA272480.13400756792592208No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG254450.125140287943155No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN249560.12273535177478388No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG246110.12103861766826439No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC245410.12069435277708652No Hit
CCCTGTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCAT245200.12059107330973315No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG231720.1139615151196222No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA213980.10523685916320025No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG208130.10235978828692807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG16350.039.57522
ACCGCGT36050.037.625586
CCGCGTT38000.036.0542347
CGCGTTC39700.034.5974968
AGACCGC39450.034.2073144
TCGACGT9700.031.39932434
CAGACCG43700.031.2764193
ATATCGC22000.030.04039421
ATACGGA45250.029.90479333
TACGGAG46250.029.33483934
AATACGG46900.028.92428832
CAATACG47850.028.71135931
GAACGAT47150.028.67979845
GCGTACG9950.028.5166532
ATCGCGG22700.028.35175123
GATATCG23850.028.00030720
CGACGTA10950.027.50067935
GCTACCT142150.026.8759751
CCGATCG357150.026.25828770
TTGCACG146900.026.213088
Produced by FastQC (version 0.11.5)