FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28104161
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG568180.20216935136402042No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG422160.15021263221485245No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA421890.1501165610316565No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA419810.14937645710185052No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC348750.12409194496145963No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC339460.12078638462112426No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG338560.12046614734380437No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA323770.1152035814198474No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT305760.10879527768147927No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT303250.10790217149695379No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC301590.10731151162989708No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT294150.1046642168040526No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG287760.10239053213508134No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGGAGGGGTGA286220.10184257057166732No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGT284590.10126258528052128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGGCG202700.032.1367571
TGGCGCG238600.027.096183
GGTCGAC89300.025.0135481
TATCGCG9400.024.31029722
GGCGCGT271650.023.7708554
GTGGCGC281400.023.0914342
TCGCACG24100.021.26457827
AGGTCGA63100.021.118476
TAGGGGG55400.020.9108738
GCGTGCC318700.020.267647
GTCGACC111950.019.8837182
ATAGGGG42750.019.87344637
TATACGC15300.019.4572147
GGCGATC28650.019.3333052
CGCACGA26800.019.25169628
TATAGGG36450.019.2151436
TCGACCC118250.019.170873
CGCGTGC335600.019.1523046
GGGCGAT29800.018.9425471
CGTGCCT340900.018.7046748
Produced by FastQC (version 0.11.5)