FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005432967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586021970
Sequences flagged as poor quality0
Sequence length16-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGG14783720.25227245319829905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC7499850.011.27651645
TACACCG340350.09.8585095
TTATACA9343100.09.47521445
TATACTG827500.09.4630025
ATACACA6773800.09.25506245
TACACTG1340350.09.1257595
TACACAT4519750.08.99674445
TAGACCG273500.08.9791145
GTGTAGG1198050.08.8418011
GTATAGG600300.08.5663311
CTACACT1239800.08.547164
CCTACAC1191950.08.4318163
GTCCTAG1199600.08.3521981
TAGACTG1065150.08.2342115
GTGTTAG1206400.08.2286691
CCTATAC610650.08.2162943
TAGACAG1664950.08.1813415
ACACATC4035800.08.10578945
CTTATAC10873200.08.10330644
CTAGGAC1232750.08.101673
Produced by FastQC (version 0.11.5)