FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005432970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005432970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216237242
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTGCGT6045506227.957747444818036No Hit
TGTGTGAC5303795224.527667625357523Illumina PCR Primer Index 5 (100% over 8bp)
CTCCAACA5087648723.528087266299856No Hit
ACAACTTG4771982922.068274899658586No Hit
GGTGTGAC4841280.22388742823495686No Hit
GTGTGCGT3394480.1569794346526118No Hit
ACAACTTA2570640.11888053955109176No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)