FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005438281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005438281
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences23174646
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT207250.013.2585991
CGCTAAT8250.012.9873761
CGGGTAA14600.012.78941231
GTTTAAT72700.011.821171
TACGCCG23400.011.78284559
GTCGATT10250.011.7599111
CGCAAAT18700.011.5788181
CGAAAAT29800.011.0112131
GTTTTAT123750.010.9671171
GTTTTCT147650.010.9455911
CTCGAAC26200.010.9054311
GTATACG14650.010.88573221
GTTTGAT73200.010.856081
GGAAAAT138900.010.6869441
CGGAAAT22950.010.6017591
GTCGAAT8250.010.5522431
CTCGAAT24450.010.4991251
CGCGAAT4701.8189894E-1210.448621
GTTTTTC114950.010.447381
TATACGG15200.010.34881322
Produced by FastQC (version 0.11.5)